ORIGINAL RESEARCH
Efficacy of Systemic Methylprednisolone and Intratympanic Dexamethasone in Rats with Noise İnduced Hearing Loss
Gürültüye Bağlı İşitme Kaybı Olan Sıçanlarda Sistemik Metilprednizolon ve İntratimpanik Dexametazonun Etkinliği
Received Date : 25 Nov 2021
Accepted Date : 22 Feb 2022
Available Online : 10 Mar 2022
Sebla ÇALIŞKANa, Murat TOPDAĞb, Kadri İLAc, Ahmet KARAd
aDepartment of Otorhinolaryngology, Head and Neck Surgery, University of Health Sciences Derince Training and Research Hospital, Kocaeli, Türkiye
bClinic of Otorhinolaryngology, Head and Neck Surgery, Altunizade Acibadem Hospital, İstanbul, Türkiye
cDepartment of Otorhinolaryngology, Head and Neck Surgery, University of Health Sciences Ümraniye Training and Research Hospital, İstanbul, Türkiye
dDepartment of Otorhinolaryngology, Head and Neck Surgery, Sakarya University Faculty of Medicine, Sakarya, Türkiye
Doi: 10.24179/kbbbbc.2021-85880 - Makale Dili: EN
KBB ve BBC Dergisi. 2022;30(2):70-7
Copyright © 2020 by Turkey Association of Society of Ear Nose Throat and Head Neck Surgery. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
ABSTRACT
Objective: To evaluate the audiological and electron microscopic
(EM) effects of systemic methylprednisolone and intratympanic
dexamethasone (IT DXM) in rats with noise induced hearing loss.
Material and Methods: Thirty-one adult female Wistar albino rats
were randomized into 3 groups as Group I (n=8), Group II (n=11) and
Group III (control, n=12). The animals were exposed to white noise at
a frequency of 1-10 kHz and 110 dB sound pressure level for 8 hours
in a free environment. Distortion product otoacoustic emission
(DPOAE) measurements were performed before and after the noise.
Group I received 0.8 mg/day IT DXM and Group II had 1 mg/kg/day
intraperitoneal methylprednisolone for 7 days. Intraperitoneal saline
solution was administered to Group III. DPOAE tests were repeated on
the 7th and 21st days. After animals were acrified on the 21st day, their
cochleas were examined under EM. Statistical analysis was performed
for the DPOAE measurements. Results: Measurement of the first day
to 7th and 21st days were compared in all groups separately. Statistical
significant recovery was observed in the frequencies of 5,000-6,000 Hz
(p<0.05) in Group I and in the frequencies of 6,000-8,000 Hz (p<0.05)
in Group II. The measurements were similar in control group for all
frequencies on the 1st, 7th and 21st days (p>0.05). Groups I and II showed
more stable and larger number of stereocilia compared to Group III on
EM examination. Conclusion: IT DXM and systemic methylprednisolone
are effective at certain frequencies in noise induced hearing
loss. The combined use of both methods may provide additional benefits.
Keywords: Hearing loss, noise-induced; injection, intratympanic; otoacoustic emissions; spontaneous
ÖZET
Amaç: Çalışmamızda, gürültüye bağlı işitme kaybı gelişen sıçanlarda,
sistemik metilprednizolon ve intratimpanik (İT) deksametazonun
odyolojik ve elektron mikroskopik (EM) etkilerinin değerlendirilmesi
amaçlandı. Gereç ve Yöntemler: Otuz bir adet erişkin dişi
Wistar albino sıçan Grup I (n=8), Grup II (n=11) ve Grup III (kontrol,
n=12) olmak üzere 3 gruba randomize edildi. Tüm sıçanlara 1-10 kHz
frekansında, 110 dB şiddetinde 8 saat boyunca kesintisiz, homojen karakterde
gürültü verildi. Gürültü öncesi ve 1 gün sonrası “distortion product
otoakustik emisyon (DPOAE)” ölçümü yapıldı. Birinci gruba 7 gün
boyunca 0,8 mg gün İT deksametazon, 2. gruba 7 gün 1 mg/kg/gün intraperitoneal
metilprednizolon uygulandı. Üçüncü gruba ise 7 gün boyunca
intraperitoneal yolla serum fizyolojik verildi. DPOAE ölçümleri 7
ve 21. günde tekrarlandı. Sıçanlar 21. günde sakrifiye edilerek kokleaları
çıkartıldı ve EM inceleme yapıldı. Ölçümler ise istatistiksel analize tabi
tutuldu. Bulgular: Tüm grupların 1. gün ölçümü ile 7 ve 21. gün ölçümleri
ayrı ayrı karşılaştırıldı. Grup I’de 5.000 ile 6.000 Hz frekanslarda istatistiksel
anlamlı iyileşme gözlendi (p<0,05). Grup II’de ise 6.000 ile 8.000
Hz frekanslarda istatistiksel anlamlı iyileşme gözlendi (p<0,05). Kontrol
grubunda ise 1. gün ile 7 ve 21. gün ölçümleri benzer olarak izlendi
(p>0,05). Elektron mikroskopide Grup I ve Grup II’de Grup III’e göre
stereosilya sayısı ve bütünlüğü kalitatif olarak daha fazla izlendi. Sonuç:
İT deksametazon ve sistemik metilprednizolon gürültüye bağlı işitme
kaybı tedavisinde belirli frekanslarda etkilidir. Her iki metodun kombine
kullanımı ek yarar sağlayabilir.
Anahtar Kelimeler: İşitme kaybı, gürültüye bağlı; enjeksiyon, intratimpanik; otoakustik emisyonlar; spontan
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