ISSN: 1300 - 6525 E-ISSN: 2149 - 0880
kulak burun boğaz
ve baş boyun cerrahisi dergisi
http://dergi.kbb-bbc.org.tr
Koşulsuz Destek Verenler
Kayıtlı İndeksler





ORIGINAL RESEARCH

Efficacy of Systemic Methylprednisolone and Intratympanic Dexamethasone in Rats with Noise İnduced Hearing Loss
Gürültüye Bağlı İşitme Kaybı Olan Sıçanlarda Sistemik Metilprednizolon ve İntratimpanik Dexametazonun Etkinliği
Received Date : 25 Nov 2021
Accepted Date : 22 Feb 2022
Available Online : 10 Mar 2022
Doi: 10.24179/kbbbbc.2021-85880 - Makale Dili: EN
KBB ve BBC Dergisi. 2022;30(2):70-7
Copyright © 2020 by Turkey Association of Society of Ear Nose Throat and Head Neck Surgery. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
ABSTRACT
Objective: To evaluate the audiological and electron microscopic (EM) effects of systemic methylprednisolone and intratympanic dexamethasone (IT DXM) in rats with noise induced hearing loss. Material and Methods: Thirty-one adult female Wistar albino rats were randomized into 3 groups as Group I (n=8), Group II (n=11) and Group III (control, n=12). The animals were exposed to white noise at a frequency of 1-10 kHz and 110 dB sound pressure level for 8 hours in a free environment. Distortion product otoacoustic emission (DPOAE) measurements were performed before and after the noise. Group I received 0.8 mg/day IT DXM and Group II had 1 mg/kg/day intraperitoneal methylprednisolone for 7 days. Intraperitoneal saline solution was administered to Group III. DPOAE tests were repeated on the 7th and 21st days. After animals were acrified on the 21st day, their cochleas were examined under EM. Statistical analysis was performed for the DPOAE measurements. Results: Measurement of the first day to 7th and 21st days were compared in all groups separately. Statistical significant recovery was observed in the frequencies of 5,000-6,000 Hz (p<0.05) in Group I and in the frequencies of 6,000-8,000 Hz (p<0.05) in Group II. The measurements were similar in control group for all frequencies on the 1st, 7th and 21st days (p>0.05). Groups I and II showed more stable and larger number of stereocilia compared to Group III on EM examination. Conclusion: IT DXM and systemic methylprednisolone are effective at certain frequencies in noise induced hearing loss. The combined use of both methods may provide additional benefits.
ÖZET
Amaç: Çalışmamızda, gürültüye bağlı işitme kaybı gelişen sıçanlarda, sistemik metilprednizolon ve intratimpanik (İT) deksametazonun odyolojik ve elektron mikroskopik (EM) etkilerinin değerlendirilmesi amaçlandı. Gereç ve Yöntemler: Otuz bir adet erişkin dişi Wistar albino sıçan Grup I (n=8), Grup II (n=11) ve Grup III (kontrol, n=12) olmak üzere 3 gruba randomize edildi. Tüm sıçanlara 1-10 kHz frekansında, 110 dB şiddetinde 8 saat boyunca kesintisiz, homojen karakterde gürültü verildi. Gürültü öncesi ve 1 gün sonrası “distortion product otoakustik emisyon (DPOAE)” ölçümü yapıldı. Birinci gruba 7 gün boyunca 0,8 mg gün İT deksametazon, 2. gruba 7 gün 1 mg/kg/gün intraperitoneal metilprednizolon uygulandı. Üçüncü gruba ise 7 gün boyunca intraperitoneal yolla serum fizyolojik verildi. DPOAE ölçümleri 7 ve 21. günde tekrarlandı. Sıçanlar 21. günde sakrifiye edilerek kokleaları çıkartıldı ve EM inceleme yapıldı. Ölçümler ise istatistiksel analize tabi tutuldu. Bulgular: Tüm grupların 1. gün ölçümü ile 7 ve 21. gün ölçümleri ayrı ayrı karşılaştırıldı. Grup I’de 5.000 ile 6.000 Hz frekanslarda istatistiksel anlamlı iyileşme gözlendi (p<0,05). Grup II’de ise 6.000 ile 8.000 Hz frekanslarda istatistiksel anlamlı iyileşme gözlendi (p<0,05). Kontrol grubunda ise 1. gün ile 7 ve 21. gün ölçümleri benzer olarak izlendi (p>0,05). Elektron mikroskopide Grup I ve Grup II’de Grup III’e göre stereosilya sayısı ve bütünlüğü kalitatif olarak daha fazla izlendi. Sonuç: İT deksametazon ve sistemik metilprednizolon gürültüye bağlı işitme kaybı tedavisinde belirli frekanslarda etkilidir. Her iki metodun kombine kullanımı ek yarar sağlayabilir.
KAYNAKLAR
  1. Nelson DI, Nelson RY, Concha-Barrientos M, Fingerhut M. The global burden of occupational noise-induced hearing loss. Am J Ind Med. 2005;48(6):446-58. [Crossref]  [PubMed] 
  2. Henderson D, Bielefeld EC, Harris KC, Hu BH. The role of oxidative stress in noise-induced hearing loss. Ear Hear. 2006;27(1):1-19. [Crossref]  [PubMed] 
  3. Wang Y, Hirose K, Liberman MC. Dynamics of noise-induced cellular injury and repair in the mouse cochlea. J Assoc Res Otolaryngol. 2002;3(3):248-68. [Crossref]  [PubMed]  [PMC] 
  4. Jin DX, Lin Z, Lei D, Bao J. The role of glucocorticoids for spiral ganglion neuron survival. Brain Res. 2009;1277:3-11. [Crossref]  [PubMed]  [PMC] 
  5. Rarey KE, Curtis LM. Receptors for glucocorticoids in the human inner ear. Otolaryngol Head Neck Surg. 1996;115(1):38-41. [Crossref]  [PubMed] 
  6. Dodson KM, Sismanis A. Intratympanic perfusion for the treatment of tinnitus. Otolaryngol Clin North Am. 2004;37(5):991-1000. [Crossref]  [PubMed] 
  7. Dodson KM, Woodson E, Sismanis A. Intratympanic steroid perfusion for the treatment of Ménière's disease: a retrospective study. Ear Nose Throat J. 2004;83(6):394-8. [Crossref]  [PubMed] 
  8. Le Prell CG, Yamashita D, Minami SB, Yamasoba T, Miller JM. Mechanisms of noise-induced hearing loss indicate multiple methods of prevention. Hear Res. 2007;226(1-2):22-43. [Crossref]  [PubMed]  [PMC] 
  9. Arts HA, Sensorineural Hearing Loss in Adults. In: Flint PW, Haughey BH, Lund V, Niparko JK, Robbins KT, Thomas JR, Lesperance MM, eds Cummings Ototlaryngology Head and Neck Surgery. 6th ed. Philadelphia: Saunders; 2015. p.2325.
  10. Spoendlin H. Primary structural changes in the organ of Corti after acoustic overstimulation. Acta Otolaryngol. 1971;71(2):166-76. [Crossref]  [PubMed] 
  11. Liberman MC, Mulroy MI. Acute and chronic effects of acoustic trauma: cochlear pathology and auditory nerve patholophysiology. In: Hamernik RP, Henderson D, Salvi R, eds. New Perspectives on Noise-İnduced Hearing Loss. New York: Raven; 1982. p.105-36.
  12. Robertson D. Functional significance of dendritic swelling after loud sounds in the guinea pig cochlea. Hear Res. 1983;9(3):263-78. [Crossref]  [PubMed] 
  13. Liberman MC, Dodds LW. Single-neuron labeling and chronic cochlear pathology. III. Stereocilia damage and alterations of threshold tuning curves. Hear Res. 1984;16(1):55-74. [Crossref]  [PubMed] 
  14. Anniko M. R Bárány. Die Beeinflussung des Ohrensausens durch intravenös injizierte Lokalanästhetica. Vorläufige Mitteilung. Acta Oto-Laryngol 1936;23:201-203. Acta Otolaryngol. 2018;138(3):247-50. [Crossref]  [PubMed] 
  15. Schuknecht HF. Ablation therapy for the relief of Ménière's disease. Laryngoscope. 1956;66(7):859-70. [Crossref]  [PubMed] 
  16. Zhou Y, Zheng H, Shen X, Zhang Q, Yang M. Intratympanic administration of methylprednisolone reduces impact of experimental intensive impulse noise trauma on hearing. Acta Otolaryngol. 2009;129(6):602-7. [Crossref]  [PubMed] 
  17. Kopke RD, Weisskopf PA, Boone JL, Jackson RL, Wester DC, Hoffer ME, et al. Reduction of noise-induced hearing loss using L-NAC and salicylate in the chinchilla. Hear Res. 2000;149(1-2):138-46. [Crossref]  [PubMed] 
  18. Bas E, Martinez-Soriano F, Láinez JM, Marco J. An experimental comparative study of dexamethasone, melatonin and tacrolimus in noise-induced hearing loss. Acta Otolaryngol. 2009;129(4):385-9. [Crossref]  [PubMed] 
  19. Seidman MD, Tang W, Bai VU, Ahmad N, Jiang H, Media J, et al. Resveratrol decreases noise-induced cyclooxygenase-2 expression in the rat cochlea. Otolaryngol Head Neck Surg. 2013;148(5):827-33. [Crossref]  [PubMed] 
  20. Rarey KE, Curtis LM, ten Cate WJ. Tissue specific levels of glucocorticoid receptor within the rat inner ear. Hear Res. 1993;64(2):205-10. [Crossref]  [PubMed] 
  21. ten Cate WJ, Curtis LM, Small GM, Rarey KE. Localization of glucocorticoid receptors and glucocorticoid receptor mRNAs in the rat cochlea. Laryngoscope. 1993;103(8):865-71. [Crossref]  [PubMed] 
  22. Doyle KJ, Bauch C, Battista R, Beatty C, Hughes GB, Mason J, et al. Intratympanic steroid treatment: a review. Otol Neurotol. 2004;25(6):1034-9. [Crossref]  [PubMed] 
  23. Parnes LS, Sun AH, Freeman DJ. Corticosteroid pharmacokinetics in the inner ear fluids: an animal study followed by clinical application. Laryngoscope. 1999;109(7 Pt 2):1-17. [Crossref]  [PubMed] 
  24. Goksu N, Haziroglu R, Kemaloglu Y, Karademir N, Bayramoglu I, Akyildiz N. Anatomy of the guinea pig temporal bone. Ann Otol Rhinol Laryngol. 1992;101(8):699-704. [Crossref]  [PubMed] 
  25. Ozdogan F, Ensari S, Cakir O, Ozcan KM, Koseoglu S, Ozdas T, et al. Investigation of the cochlear effects of intratympanic steroids administered following acoustic trauma. Laryngoscope. 2012;122(4):877-82. [Crossref]  [PubMed] 
  26. Takemura K, Komeda M, Yagi M, Himeno C, Izumikawa M, Doi T, et al. Direct inner ear infusion of dexamethasone attenuates noise-induced trauma in guinea pig. Hear Res. 2004;196(1-2):58-68. [Crossref]  [PubMed] 
  27. Takahashi K, Kusakari J, Kimura S, Wada T, Hara A. The effect of methylprednisolone on acoustic trauma. Acta Otolaryngol. 1996;116(2):209-12. [Crossref]  [PubMed] 
  28. Chandrasekhar SS, Rubinstein RY, Kwartler JA, Gatz M, Connelly PE, Huang E, et al. Dexamethasone pharmacokinetics in the inner ear: comparison of route of administration and use of facilitating agents. Otolaryngol Head Neck Surg. 2000;122(4):521-8. [Crossref]  [PubMed] 
  29. Kopke RD, Hoffer ME, Wester D, O'Leary MJ, Jackson RL. Targeted topical steroid therapy in sudden sensorineural hearing loss. Otol Neurotol. 2001;22(4):475-9. [Crossref]  [PubMed] 
  30. Sennaroglu L, Sennaroglu G, Gursel B, Dini FM. Intratympanic dexamethasone, intratympanic gentamicin, and endolymphatic sac surgery for intractable vertigo in Meniere's disease. Otolaryngol Head Neck Surg. 2001;125(5):537-43. [Crossref]  [PubMed]