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ORIGINAL RESEARCH
Effects of Insulin-Like Growth Factor-1 and Platelet-Rich Plasma on Facial Nerve Injury in a Rat Model
Rat Modelinde İnsülin Benzeri Büyüme Faktörü-1 ve Plateletten Zengin Plazmanın Fasiyal Sinir Yaralanması Üzerine Etkileri
Received Date : 17 Mar 2022
Accepted Date : 15 Jun 2022
Available Online : 23 Jun 2022
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Cemile AÇIKGÖZ YILDIZa, Necmi ARSLANa, Muzaffer ÇAYDEREb, Mehmet ŞENEŞc, Şule DEMİRCİa, Cevdet YILMAZc
aDepartment of Otorinolaryngology-Head and Neck Surgery, Health Sciences University, Ankara Training and Research Hospital, Ankara, Türkiye bDepartment of Pathology, Health Sciences University, Ankara Training and Research Hospital, Ankara, Türkiye cDepartment of Biochemistry, Health Sciences University, Ankara Training and Research Hospital, Ankara, Türkiye
Doi: 10.24179/kbbbbc.2022-89683 - Makale Dili: EN
KBB ve BBC Dergisi. 2022;30(3):154-64
Copyright © 2020 by Turkey Association of Society of Ear Nose Throat and Head Neck Surgery. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
ABSTRACT
Objective: Insulin-like growth factor-1 (IGF-1) and platelet rich plasma (PRP) on nerve healing to evaluate electrophysiologically and histopathologically by experimental traumatic peripheral facial paralysis in rats. Material and Methods: Three groups of 7 rats in each were formed. Group 1: Control group. Group 2: Local IGF-1 applied. Group 3: Local PRP applied. After nerve identification, neuron excitability threshold (NET), latency and amplitude values of left facial nerve buccal branch (LFNBB) were recorded. The LFNBB of all rats in all groups was cut and sutured. PRF was applied to Group 2, IGF-1 and Group 3, respectively. Control measurements of LFNBB were performed at 12 weeks. Then traumatized part of LFNBB was removed and axonal degeneration, vascular congestion and macrovacuolization were evaluated histopathologically. Results: When all groups were compared, significant difference was observed in Group 3 (p1=0.002, p2<0.001, p3=0.017 respectively) in NET. Group 2 and 3 values were significantly different than control group (p1=0.011, p2=0.007) in latency values. There was no difference between Group 2 and 3. Group 2 and 3 values were significantly different in amplitude values compared to control group (p1<0.001, p2<0.001). While there was no difference between Group 2 and 3. In all histopathological examinations, a significant improvement was observed in Group 2 and 3 compared to control group, findings of histopathological improvement in the Group 3 was superior. Conclusion: The findings obtained from this study showed that local IGF-1 and PRP application had positive effects on nerve healing in traumatic facial palsy while PRP was more effective.
Keywords: Insulin-like growth factor-1; platelet rich plasma; traumatic facial nerve paralysis
ÖZET
Amaç: Sıçanlarda deneysel travmatik periferik fasiyal paralizi ile sinir iyileşmesi üzerine insülin benzeri büyüme faktörü-1 [insulinlike growth factor-1 (IGF-1)] ve trombositten zengin plazma [platelet rich plasma (PRP)] elektrofizyolojik ve histopatolojik olarak değerlendirmektir. Gereç ve Yöntemler: Her biri 7 sıçandan oluşan 3 grup oluşturuldu. Grup 1: Kontrol grubu. Grup 2: Lokal IGF-1 uygulanan grup. Grup 3: Lokal PRP uygulanan grup. Sinir tanımlaması sonrası sol bukkal dalın nöron eksitabilite eşiği [Neuron Excitability Threshold (NET)], latans ve amplitüd değerleri kaydedildi. Her 3 gruptaki sıçanların hepsinin sol fasiyal sinirinin bukkal dalı kesilerek dikildi. Grup 2’ye IGF-1 ve Grup 3’e PRP uygulaması yapıldı. On ikinci haftada sol bukkal dalın kontrol ölçümleri yapılmıştır. Daha sonra, 12. haftada sol bukkal dalın travmatize olan kısmı çıkarıldı ve histopatolojik olarak aksonal dejenerasyon, vasküler konjesyon ve makrovakuolizasyon değerlendirildi. Bulgular: Her üç grup da kendi aralarında karşılaştırıldığında, Grup 3’te (sırasıyla p1=0,002, p2<0,001, p3=0,017) NET’te anlamlı farklılık gözlendi. On ikinci haftada kaydedilen latans değerleri, Grup 2 ve 3 değerleri kontrol grubundan anlamlı derecede farklıydı (p1=0,011, p2=0,007). Grup 2 ve 3 arasında fark yoktu. Grup 2 ve 3 değerleri kontrol grubuna göre amplitüd değerlerinde anlamlı farklılık gösterdi (p1<0,001, p2<0,001). Grup 2 ve 3 arasında fark bulunmazken, histopatolojik olarak değerlendirilen tüm incelemelerde, IGF 1 ve PRP ile tedavi edilen kontrol grubuna göre anlamlı iyileşme gözlendi, PRP ile tedavi edilen grupta histopatolojik iyileşme bulguları daha üstündü. Sonuç: Bu çalışmadan elde edilen bulgular, travmatik fasiyal felçte lokal IGF-1 ve PRP uygulamasının sinir iyileşmesi üzerinde olumlu etkilerinin olduğunu, PRP’nin ise daha etkili olduğunu göstermiştir.
Anahtar Kelimeler: İnsülin benzeri büyüme faktörü-1; trombositten zengin plazma; travmatik fasiyal sinir paralizisi
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