ORIGINAL RESEARCH
The Effects of Platelet-Rich Plasma and Platelet-Rich Fibrin on Rabbit Auricular Cartilage Regeneration
Trombositten Zengin Plazma ve Trombositten Zengin Fibrin Yapılarının Tavşan Kulak Kıkırdağı Rejenerasyonu Üzerindeki Etkilerinin Karşılaştırılması
Received Date : 30 May 2024
Accepted Date : 06 Sep 2024
Available Online : 23 Sep 2024
Ceren KARAÇAYLIa, Siyami KARAHANb, Mustafa KAZKAYASIa, Rahmi KILIÇa
aKırıkkale University Faculty of Medicine, Department of Otolaryngology, Kırıkkale, Türkiye
bKırıkkale University Faculty of Veterinary, Department of Histology, Kırıkkale, Türkiye
Doi: 10.24179/kbbbbc.2024-104117 - Makale Dili: EN
Journal of Ear Nose Throat and Head Neck Surgery. 2024;32(4):154-62.
Copyright © 2020 by Turkey Association of Society of Ear Nose Throat and Head Neck Surgery. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
ABSTRACT
Objective: This study aims to examine the impact of
platelet rich fibrin (PRF) and platelet rich plasma (PRP) on the repair
of cartilage tissue. The objective is to discover if these materials effectively
boost the healing ability of cartilage tissue. Material and Methods:
The study consisted of 18 male rabbits from New Zealand, all of
whom were adults and white in color. The rabbits were partitioned into
three study cohorts, each consisting of six rabbits. The study group was
partitioned into subgroups A and B, each consisting of three rabbits.
Cartilage of 10×10 mm2 was surgically removed from the ears of rabbits
in all a subgroups. A 1 mm section of cartilage was removed from
the outer edge of the excised cartilage to decrease its dimensions. The
8×8 mm cartilage acquired from the initial group was positioned in the
exact location from whence it was extracted. Within all B subgroups, a
section of cartilage measuring 10×10 mm2 was extracted from the ears
of rabbits. In the initial cohort, a volume of 2 milliliters of PRP was administered
onto the mucoperichondrium at the site where the cartilage
was excised. In the second group, PRF was applied to the site where the
cartilage was excised. No intervention was conducted on the area where
cartilage was removed in the third group. A scoring system was used to
evaluate degenerative changes in microscopic cartilage analysis. Results:
There was no significant difference between the groups in terms
of cartilage healing. Conclusion: PRP and PRF don’t improve healing
of the cartilage tissue in ear.
Keywords: Platelet rich plasma; platelet rich fibrin; rabbits; ear cartilage; growth factors
ÖZET
Amaç: Bu çalışmanın amacı, trombositten zengin fibrin (TZF)
ve trombositten zengin plazmanın (TZP) kıkırdak dokusunun onarımı
üzerindeki etkisini incelemek ve bu materyallerin kıkırdak dokusunun
iyileşme yeteneğini etkili bir şekilde artırıp artırmadığını araştırmaktır.
Gereç ve Yöntemler: Bu çalışmaya 18 adet beyaz erkek Yeni Zelanda
tavşanı dâhil edilmiştir. Tavşanlar her bir kohortta 6 tavşan olacak
şekilde 3 gruba ayrılmıştır. Bu 3 grup da kendi içinde her biri üçer tavşan
içerecek şekilde A ve B alt gruplarına ayrılmıştır. Tüm A alt gruplarında
tavşanların kulaklarından 10×10 mm2 kıkırdak eksize edilmiştir.
Dışarıya çıkarılan kıkırdağın çevresinden 1 mm’lik kıkırdak eksize edilmiştir.
Birinci grupta kalan 8×8 mm2 kıkırdak TZP ile muamele edildikten
sonra çıkarıldığı alana yerleştirilmiştir. İkinci grupta kıkırdak
TZF ile muamele edildikten sonra çıkarıldığı alana yerleştirilmiştir.
Üçüncü grupta ise kıkırdak herhangi bir muameleye tabi tutulmaksızın
çıkarıldığı alana yerleştirilmiştir. Tüm B alt gruplarında tavşanların kulaklarından
10×10 mm2 kıkırdak eksize edilmiştir. Eksize edilen kıkırdak
yerine yerleştirilmemiştir. Birinci grupta kıkırdağın çıkarıldığı
alanda birbiri ile karşılıklı gelen mukoperikondrium üzerine TZP uygulanmıştır.
İkinci grupta kıkırdağın çıkarıldığı alana TZF yerleştirilmiştir.
Üçüncü grupta ise kıkırdağın çıkarıldığı alana bir müdahalede
bulunulmamıştır. Mikroskobik kıkırdak analizinde dejeneratif değişiklikleri
değerlendirmek için bir skorlama sistemi kullanılmıştır. Bulgular:
Kıkırdak iyileşmesi açısından gruplar arasında anlamlı bir fark
bulunmamıştır. Sonuç: TZP ve TZF’nin kıkırdak iyileşmesi üzerine etkisi
yoktur.
Anahtar Kelimeler: Plateletten zengin plazma; plateletten zengin fibrin; tavşan; kulak kıkırdağı; büyüme faktörleri
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