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ORIGINAL RESEARCH

The Role of Cytokines in the Pathophysiology of Recurrent Tonsillitis
Rekürren Tonsillit Patofizyolojisinde Sitokinlerin Rolü
Received Date : 16 Feb 2022
Accepted Date : 17 Mar 2022
Available Online : 25 Mar 2022
Doi: 10.24179/kbbbbc.2022-88985 - Makale Dili: EN
KBB ve BBC Dergisi. 2022;30(2):83-7
Copyright © 2020 by Turkey Association of Society of Ear Nose Throat and Head Neck Surgery. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
ABSTRACT
Objectives: Recurrent tonsillitis and tonsillar hypertrophy are two diseases develop in the same tissue but most probably with different pathophysiological mechanisms. The role of many chemokines in tonsillitis is still being investigated. In this study, IL-1 α and β, IL-6, IL-8, IL-15, and TNF α expressions in chronic-inflamed and hypertrophic tonsil tissue were studied. Material and Methods: The study was conducted with Baskent University Department of Otolaryngology and Medical Genetics. A total of 45 specimens from patients with recurrent tonsillitis (17) and tonsillar hypertrophy (28) who underwent tonsillectomy were included in the study. Comparison between expression levels of cytokines and tissue groups was performed by Real Time Polymerase Chain Reaction (RT-PCR) technique. Results: We found a statistically significant difference only in the expression of IL-8. The mean expression level of IL-8 was higher in the hypertrophy group than in the other group. Conclusion: In our study, we didn’t find a significant difference in the expression levels of IL-1 α and β, IL-6, IL-15, and TNF α. Only IL-8 expression was higher in tonsillar hypertrophy group. This result is thought to be related to the increased number of germinal centers seen in the hypertrophy group. It is thought that new studies investigating these cytokines and other cytokines will guide us to find the difference between hypertrophy and recurrent/ chronic inflammation.
ÖZET
Amaç: Rekürren tonsillit ve tonsillar hipertrofi aynı dokuda muhtemel farklı patofizyolojik mekanizmalar ile gelişen farklı iki hastalıktır. Tonsillit gelişiminde kemokinlerin rolü hala araştırılmaktadır. Bu çalışmada kronik inflamasyonlu ve hipertrofik tonsil dokusunda IL-1 α ve β, IL-6, IL-8, IL-15, ve TNF α ekspresyon durumunu araştırdık. Gereç ve Yöntemler: Bu çalışma Başkent Üniversitesi Kulak Burun Boğaz ve Tıbbi Genetik Anabilim Dalları tarafından yürütüldü. Rekürren tonsillit (17) ve tonsillar hipertrofi (28) nedeni ile tonsillektomi uygulanan hastalardan elde edilen toplam 45 doku kullanılarak RT-PCR yöntemi ile doku grupları arasındaki sitokin düzeyleri karşılaştırıldı. Bulgular: İncelenen sitokinler arasında sadece IL-8 ekspresyon düzeyi açısından istatistiksel fark tespit edildi. IL-8 ekspresyon düzeyi hipertrofik tonsil grubunda daha yüksek saptandı. Sonuç: Bu çalışmada of IL-1 α ve β, IL-6, IL-15, ve TNF α ekspresyonları açısından anlamlı bir farklılık tespit etmedik. Sadece IL-8 ekspresyon düzeyinin tonsillar hipertrofi grubunda yüksek çıkmasını, hipertrofik tonsillerde germinal merkezlerin fazla olmasıyla ilişkilendirdik. Sitokinlerin tonsillit ve hipertrofi patofizyolojisindeki rollerinin araştırılması için yeni çalışmalar gereklidir.
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